Exploring HLA-DQA & HLA-DQB Polymorphisms in Local Population of Pakistan





Human leukocyte antigen, HLA polymorphism, Genetic diversity, HLA-DQA1, HLA-DQB1, Allelic frequency


Human leukocyte antigens (HLA) are pivotal in immune regulation, crucial in organ transplants. Allelic frequencies at HLA-DQA1 and HLA-DQB1 were compared across age, gender, patient/donor status, blood group, cities, and ethnicity in 100 participants. DNA extracted from blood samples underwent quantification, hybridization, and sequence-specific oligonucleotide (SSO) typing. Match IT DNA software analyzed SSO typing, and statistical tools including Student's T-test and Analysis of Variance (ANOVA) assessed data, utilizing GraphPad Prism 6 and Microsoft Excel. Findings revealed varying allele frequencies across parameters. Locally, DQA106 and DQB104 were less common, contrasting with DQA101 and DQB103 prevalence. Among ethnic groups, Punjabis showed higher DQA101 frequency, while DQB1 alleles were less common. Regarding blood groups, DQA101/02/05/06 and DQB102/04/05 were prominent in O+ individuals, with DQA103 and DQB103 in B+ individuals. Males displayed higher DQA106 and DQB103 prevalence than females. Across age groups, DQA1 dominance was observed in age group II compared to DQB1 alleles. DQB101 and DQA1*03 were more frequent in donors than patients. Due to balancing selection, DQA1 had the highest allelic frequency, while DQB1 had the lowest. This study offers valuable insights into HLA II loci allele frequencies, aiding in understanding the Pakistani population's immunogenetic landscape.


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How to Cite

Quddus, F., & Batool, S. S. (2023). Exploring HLA-DQA & HLA-DQB Polymorphisms in Local Population of Pakistan. TSF Journal of Biology , 1(1), 19–34. https://doi.org/10.69547/tsfjb.v1i1.9