Use of Industrial Wastewater for Chlorella sp. Culturing to obtain Commercially Important Compounds



Chlorella sp, industrial wastewater, GC-MS analysis, siloxanes


In the present study, Chlorella sp. was grown in volume-graded industrial wastewater and showed increased growth in 10% and 30% of wastewater but the maximum growth was shown in 100% industrial wastewater. Among nitrogen sources, maximum growth was observed in urea (6.80×105 cells/ml) while the least was determined in NH4Cl (1.40×105 cells/ml). The maximum no. of algal cells (2.50×105) was determined in 0% NaCl while the minimum cells were determined in 2% NaCl (0.002×105). The optimum algal growth was determined at pH 7 (7.0×105 cells/ml) but at pH 9 the alga showed significant growth (5.9×105 cells/ml). The alga showed resistance towards erythromycin and chloramphenicol but was sensitive against ampicillin and gentamicin. Alga showed high growth in the presence of Cd and Pb (5µg) while less growth was determined in the presence of Hg, Cu, and Cr (5µg). Different compounds including cyclotetrasiloxane, octamethyle-, cyclopentasiloxane, decamethyle-, cyclohexasiloxane, dodecamethyle-, cycloheptasiloxane, tetradecamethyle- and benzeneethaneamine were isolated and analyzed by GC-MS analysis.. Siloxanes have great significance in industrial products, especially in cosmetics and textile industries. Besides biofuel production alga has great potential to be used for various industrial products.


Blaga AC, Zaharia C, Suteu DJP. Polysaccharides as support for microbial biomass-based adsorbents with applications in removal of heavy metals and dyes. Polymers. 2021;13(17):2893.

Das SK, Ghosh GK, Avasthe RJBC. Conversion of crop, weed and tree biomass into biochar for heavy metal removal and wastewater treatment. Biomass Conversion. 2021:1-14.

Sharma P, Pandey AK, Udayan A, Kumar S. Role of microbial community and metal-binding proteins in phytoremediation of heavy metals from industrial wastewater. Bioresour Technol. 2021;326:124750.

Rue GP, Darling JP, Graham E, Tfaily MM, McKnight DM. Dynamic changes in dissolved organic matter composition in a Mountain Lake under ice cover and relationships to changes in nutrient cycling and phytoplankton community composition. Aquat Sci. 2020;82:1-16.

Cutajar J, Sterba-Boatwright B, Wetz MS. Coastal residential canals harbor distinct water quality conditions and phytoplankton community composition. Coastal Shelf Sci. 2024;296:108595.

Bandyopadhyay D, Biswas H. Impacts of variable nutrient stoichiometry (N, Si and P) on a coastal phytoplankton community from the SW Bay of Bengal, India. Eur J Phycol. 2021;56(3):273-288.

Butts TJ, Moody EK, Wilkinson GMJ. Contribution of zooplankton nutrient recycling and effects on phytoplankton size structure in a hypereutrophic reservoir. J Plankton Res. 2022;44(6):839-853.

Low SS, Bong KX, Mubashir M, et al. Microalgae cultivation in palm oil mill effluent (POME) treatment and biofuel production. Sustainability. 2021;13(6):3247.

Shahid A, Malik S, Zhu H, et al. Cultivating microalgae in wastewater for biomass production, pollutant removal, and atmospheric carbon mitigation; a review. Sci Total Environ. 2020;704:135303.

Zainith S, Saxena G, Kishor R, Bharagava RN. Application of microalgae in industrial effluent treatment, contaminants removal, and biodiesel production: Opportunities, challenges, and prospects. In: Bioremediation for environmental sustainability. 2021:481-517.

Kumar R, Ghosh AK, Pal P. Synergy of biofuel production with waste remediation along with value-added co-products recovery through microalgae cultivation: A review of membrane-integrated green approach. Sci Total Environ. 2020;698:134169.

Al-Jabri H, Das P, Khan S, Thaher M, AbdulQuadir MJW. Treatment of wastewaters by microalgae and the potential applications of the produced biomass—A review. Water. 2020;13(1):27.

Lipps WC, Baxter TE, Braun-Howland EB, Association APH, Association AWW. Standard methods for the examination of water and wastewater: American public health association. 2023.

Zarrinmehr MJ, Farhadian O, Heyrati FP, et al. Effect of nitrogen concentration on the growth rate and biochemical composition of the microalga, Isochrysis galbana. Egypt J Aquat Res. 2020;46(2):153-158.

Díaz S, Aguilera Á, de Figueras CG, et al. Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli. Int J Environ Res Public Health. 2022;19(13):7692.

Henson SA, Cael B, Allen SR, Dutkiewicz S. Future phytoplankton diversity in a changing climate. Nat Commun. 2021;12(1):5372.

Sathish T, Nazrin A, Thomas LC, Padmakumar K. Seasonal dynamics of dinoflagellates with special emphasis on potentially harmful species in a tropical estuarine system along the southwest coast of India. J Oceanography. 2022;78(5):397-408.

Wang Q, Chen X, Lin L, et al. The dispersal of dinoflagellate cyst caused by international ships under repair conditions: a potential invasion risk to the Yangtze River Estuary, China. Environ Sci Pollut Res. 2023;30(36):86178-86188.

Mwaurah PW, Kumar S, Kumar N, et al. Novel oil extraction technologies: Process conditions, quality parameters, and optimization. Compr Rev Food Sci Food Saf. 2020;19(1):3-20.

Ok JH, Jeong HJ, You JH, et al. Phytoplankton bloom dynamics in incubated natural seawater: predicting bloom magnitude and timing. Front Mar Sci. 2021;8:681252.

Berg H, Belt S, Koole S, Van der Wulp N. Environmental preference and restoration: (How) are they related?. J Environ Psychol. 2003;23(2):23. doi:

Guo Q, Li T, Qu Y, et al. New research development on trans fatty acids in food: Biological effects, analytical methods, formation mechanism, and mitigating measures. Prog Lipid Res. 2023;89:101199.

Kaseke T, Opara UL, Fawole OA. Fatty acid composition, bioactive phytochemicals, antioxidant properties and oxidative stability of edible fruit seed oil: Effect of preharvest and processing factors. Heliyon. 2020;6(9).

Alazaiza MY, Albahnasawi A, Al Maskari T, Abujazar MSS, Bashir MJ, Nassani DE, Abu Amr SSJE. Biofuel production using cultivated algae: Technologies, economics, and its environmental impacts. Energies. 2023;16(3):1316.

Quero-Jiménez P, Felipe L, López LJ. Oil extraction and derivatization method: A review. Open Access J Sci. 2020;4:110-120.

Hossain MS, Mohamed SH, Khalid AMB, et al. Avenues in supercritical carbon dioxide extraction and fractionation of lipids. In: Innovative Food Processing Technologies. Elsevier; 2021:584-596.




How to Cite

Afzal, G., Bukhari, D. A., & Rehman, A. (2023). Use of Industrial Wastewater for Chlorella sp. Culturing to obtain Commercially Important Compounds. TSF Journal of Biology , 1(2), 63–75. Retrieved from