Strong Cation Exchange Chromatography-based Bioactivity of Cationic Peptides from Solanum lycopersicum (Tomato) Under Salt Stress

SCX Chromatography-based Bioactivity of Peptides from tomato

Authors

  • Nazia Kanwal Department of Biological Sciences, Superior University, Lahore, Pakistan
  • Aisha Tahir Department of Biochemistry, University of Health Sciences, Lahore, Pakistan
  • Asma Noreen Department of Botany, University of Agriculture, Faisalabad, Pakistan
  • Alishba Mumtaz Department of Biological Sciences, Superior University, Lahore, Pakistan
  • Amer Jamil Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan

DOI:

https://doi.org/10.69547/NS.22.04

Keywords:

Cationic peptides, Antibacterial, Saline stress, Mass Spectrometry, Abiotic stress

Abstract

The rise in antibiotic resistance in pathogens has led to an exponential rise in infectious diseases. It has alarmed the need for new antibiotics with precise actions against pathogens and ability to target multi directionally to encounter disease causing agents. Cationic peptides are also known as natural antibiotics and play a fundamental role in recruiting and promoting agents of innate as well as adaptive immune systems. These are produced constitutively as well as under the influence of biotic and abiotic stresses. In the current study, Solanum lycopersicum (tomato) was induced to express cationic proteins/ peptides under salt stress. Proteins up to molecular mass of 10 kDa were isolated and fractionated by cation exchange chromatography followed by antibacterial assays. The results showed that tomato plants could tolerate salt stress of 200 mM, where 100 mM of stress provoked more antibacterial peptides/ proteins. Strong cation exchange fractionation separated enormous positively charged peptides which showed high percentage of antibacterial activity. The prolific antimicrobial peptides authenticate the tomato plant as a promising candidate for pharmaceuticals, nutraceuticals and drug discovery.

Additional Files

Published

2024-12-07

How to Cite

Kanwal, N., Tahir, A., Noreen, A., Mumtaz, A., & Jamil, A. (2024). Strong Cation Exchange Chromatography-based Bioactivity of Cationic Peptides from Solanum lycopersicum (Tomato) Under Salt Stress: SCX Chromatography-based Bioactivity of Peptides from tomato. Nature’s Symphony , 2(2), 37–52. https://doi.org/10.69547/NS.22.04

Issue

Vol. 2 No. 2 (2024): Nature's Symphony-Volume 2, Issue 2

Section

Chemistry

License

Copyright (c) 2024 Nazia Kanwal, Aisha Tahir, Asma Noreen, Alishba Mumtaz, Amer Jamil

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