Surveillance of Mycoplasma gallisepticum in Bird’s Retail Stalls in Quetta District

Surveillance of Mycoplasma Gallisepticum in Birds

Abstract

One of the most economically significant and contagious Mycoplasma species is Mycoplasma gallisepticum, a significant pathogen of poultry production facilities causing high economic losses in Pakistan. Most countries rely on M. gallisepticum control efforts to maintain disease-free commercial breeding stock.  Molecular biology techniques have been applied for the quick diagnosis of these infections and the implementation of disease control measures. A rapid and precise alternative to traditional culture techniques is the polymerase chain reaction (PCR). A single tube reaction was devised for the entire assay (Multiplex MG), which was designed with primers and probes unique for each pathogen. The aim of this project was to assess the molecular technique for Mycoplasma gallisepticum detection in bird trachea. 22 tracheal samples in total were processed using the PCR method of detection to examine the disease's spread inside the sample. 10 out of the 22 tracheal samples that were examined by PCR were positive and 12 were negative. The findings of this study showed that presence of Mycoplasma gallisepticum were detected in the samples. Additionally, this investigation demonstrated that Mycoplasma gallisepticum is still present in commercial poultry and should be controlled by novel preventative measures based on drugs and immunizations, likely employing new vaccines depending on the local Mycoplasma gallisepticum strain's characteristics.