Isolation and Growth Optimization of Mercury-Resistant Bacteria Isolated from River Ravi
Isolation and Growth Optimization of Mercury-Resistant Bacteria
DOI:
https://doi.org/10.69547/NS.12.02Keywords:
Mercury, Wastewater, Bioremediation, Mercury reduction, MICAbstract
Mercury (Hg) is one of the most toxic heavy metals found on earth and has been reported as a contaminant from industrial and agricultural activities. Its release in the environment has led to extreme toxicity to all life forms. Various strategies like physico-chemical techniques have been proposed for Hg removal; bioremediation has proved to be most promising among all. This study focuses on the isolation of Hg resistant bacteria from Ravi River samples. Several biochemical tests were performed like gram staining, catalase, oxidase, sugar fermentation, antibiotic resistance, and motility test. Minimum inhibitory concentration (MIC) displayed that among all the isolates, Hg4 was able to resist up to 2500 ppm Hg followed by Hg6 and Hg7 which showed resistance till 2000 ppm Hg. These isolates were subjected to mercury reduction assay. Results revealed that Hg4, Hg6, and Hg7 bioremediated 59.82%, 40.82%, and 65.00% of mercury (Hg) respectively after 24 hours. In future, these isolates can be used for the bioremediation of Hg from contaminated sites which will help in creating a healthy environment.
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Copyright (c) 2023 Muhammad Ihtasham , Shahzara Irshad, Hareem Mohsin

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